General description

Histones are small alkaline proteins that interact with eukaryotic DNA to form the fundamental subunit of chromatin known as the nucleosome. The core histones H2A, H2B, H3 and H4 form the central spooling unit of the nucleosome; with two copies of each comprising a histone octamer that interacts with the negatively charged DNA. They enable an ordered compaction of the DNA, as well as spatiotemporal control of gene expression and genomic replication throughout mitosis. Post-translational modification of this core complex results in structural changes within the chromatin and epigenetic control of gene expression. 

Principle

The Core Histone Isolation Kit offers an optimized suite of reagents to rapidly extract the total core histones, while preserving acetylation from tissues and cultured cells for biochemical and epigenetic analyses. One hundred standard extractions at a scale of 10⁷ cells or 100 mg of tissue can be performed with the reagents in this kit. Yield of the total histone proteins is ~0.4 mg per 10⁷ cells or 100 mg of tissue.

Suitability

Suitable for the isolation of intact functional core histones from tissue and cultured cells, protein profiling, post-translational modification and epigenetic analyses and Western blot and ELISA